{"id":427,"date":"2026-08-18T13:08:52","date_gmt":"2026-08-18T13:08:52","guid":{"rendered":"https:\/\/omivera.jppwebdesign.nl\/?page_id=427"},"modified":"2026-10-26T10:21:18","modified_gmt":"2026-10-26T10:21:18","slug":"covalent-inhibitors","status":"publish","type":"page","link":"https:\/\/omivera.jppwebdesign.nl\/?page_id=427","title":{"rendered":"Covalent Inhibitors"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.27.6″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”122deg” background_color_gradient_stops=”#001f52 0%|rgba(235,242,255,0.03) 99%” background_color_gradient_overlays_image=”on” background_image=”https:\/\/omivera.jppwebdesign.nl\/wp-content\/uploads\/2026\/06\/omi_Website-Omivera-9.png” background_enable_video_mp4=”off” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.27.6″ _module_preset=”default” min_height=”50vh” custom_css_main_element=”border-bottom: 5px solid;||border-image-slice: 1;||border-image-source: linear-gradient(|| 90deg,|| #ebf2ff 0%,|| #228cdb 50%,|| #001f52 100%||);||border-top: 0;||border-left: 0;||border-right: 0;” border_width_bottom=”5px” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.9″ _module_preset=”default” header_font=”Hanken|300|||||||” header_text_color=”#FFFFFF” header_font_size=”40px” header_2_font=”–et_global_heading_font|100|||||||” header_2_text_color=”#B8DAF4″ header_2_font_size=”36px” header_2_line_height=”1.2em” global_colors_info=”{}”]<\/p>\n

Tailored chemical proteomics for covalent inhibitors<\/h2>\n

Turn your covalent inhibitor into a chemical probe for target engagement and selectivity studies.<\/h1>\n

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Starting from your existing compound, Omivera can help develop a tailored chemical probe that enables you determine where in a biological system and which proteins your inhibitor engages. For a covalent inhibitor, a probe can be built directly from the compound itself by introducing a small bio-orthogonal handle at a position where structure-activity relationship data suggest it will not interfere with binding or reactivity. The warhead, already present in the covalent inhibitor, allows for binding the intended target as well as potential off-targets.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” background_image=”https:\/\/omivera.jppwebdesign.nl\/wp-content\/uploads\/2026\/06\/omi_Website-Omivera-18.png” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” background_enable_color=”off” custom_padding=”||||true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.6″ _module_preset=”default” text_font=”Hanken||||||||” text_text_color=”#FFFFFF” header_2_font=”Hanken||||||||” header_2_font_size=”32px” header_3_font=”Hanken|200|||||||” background_color=”#001F52″ background_layout=”dark” custom_margin=”||||false|false” custom_padding=”3vh|2vw|3vh|2vw|true|true” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”]<\/p>\n

1. Start with your covalent inhibitor<\/span><\/h3>\n

We begin with the compound you want to investigate and assess how it can be adapted for chemical proteomics.<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.6″ _module_preset=”default” text_font=”Hanken||||||||” text_text_color=”#FFFFFF” header_2_font=”Hanken||||||||” header_2_font_size=”32px” header_3_font=”Hanken|200|||||||” background_color=”#228CDB” background_layout=”dark” custom_padding=”3vh|2vw|3vh|2vw|true|true” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”]<\/p>\n

2. Add a click handle<\/span><\/h3>\n

A reporter handle is introduced to create a chemical probe while retaining the relevant properties of the original inhibitor.<\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.6″ _module_preset=”default” text_font=”Hanken||||||||” text_text_color=”#FFFFFF” header_2_font=”Hanken||||||||” header_2_font_size=”32px” header_3_font=”Hanken|200|||||||” background_color=”#6facdb” background_layout=”dark” custom_padding=”3vh|2vw|3vh|2vw|true|true” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”]<\/p>\n

3. Study target engagement<\/span><\/h3>\n

The probe can then be used to measure target engagement and compound selectivity in the biological system relevant to your program.<\/span><\/p>\n

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4. Choose the right readout<\/span><\/h3>\n

Depending on the question, studies can use either gel-based profiling or mass-spectrometry-based chemical proteomics.<\/span><\/p>\n

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What questions can this approach answer?<\/h2>\n

\u2713 What is the target engagement of the covalent inhibitor?<\/p>\n

\u2713 What is the full set of proteins that your covalent inhibitor reacts with?<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.27.6″ _module_preset=”default” background_image=”https:\/\/omivera.jppwebdesign.nl\/wp-content\/uploads\/2026\/06\/omi_Website-Omivera-11.png” border_radii=”on|1vh|1vh|1vh|1vh” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,1_3,1_3″ _builder_version=”4.27.6″ _module_preset=”default” background_color=”#EBF2FF” custom_padding=”3vh|2vw|3vh|2vw|true|true” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.27.6″ _module_preset=”default” custom_padding=”|1vw||0vw|false|false” border_color_all=”#FFFFFF” border_width_right=”2px” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.9″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n\n\n\n\n\n
\n

Model<\/strong><\/span><\/h3>\n<\/td>\n<\/tr>\n<\/thead>\n

Project dependent: live cells, cell lysates, or tissues<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.27.6″ _module_preset=”default” custom_padding=”|1vw|||false|false” border_width_right=”2px” border_color_right=”#FFFFFF” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.9″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n\n\n\n\n\n
\n

Targets<\/strong><\/span><\/h3>\n<\/td>\n<\/tr>\n<\/thead>\n

Any proteins with a reactive residue that covalently interacts with the inhibitor\u2019s warhead<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.27.6″ _module_preset=”default” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.9″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n\n\n\n\n\n
\n

Best suited for<\/strong><\/span><\/h3>\n<\/td>\n<\/tr>\n<\/thead>\n

Target engagement & off-target mapping for covalent inhibitors<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ disabled_on=”on|on|on” _builder_version=”4.27.6″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_stops=”#228cdb 1%|#b8daf4 98%” background_image=”https:\/\/omivera.jppwebdesign.nl\/wp-content\/uploads\/2026\/07\/omi_Website-Omivera-41.png” parallax=”on” background_blend=”multiply” min_height=”30vh” custom_padding=”||||false|false” disabled=”on” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.6″ _module_preset=”default” text_font=”Hanken|300|||||||” text_text_color=”#FFFFFF” background_color=”#001F52″ background_layout=”dark” custom_padding=”3vh|2vw|3vh|2vw|true|true” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”]<\/p>\n

Built around your compound<\/strong><\/h3>\n

Tailored chemical proteomics is particularly useful when an off-the-shelf probe or assay is not available.<\/p>\n

By developing the workflow around your inhibitor, we can create a target engagement approach that fits the specific biology and questions of your program.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.6″ _module_preset=”default” text_font=”Hanken|300|||||||” text_text_color=”#FFFFFF” background_color=”#228CDB” background_layout=”dark” custom_padding=”3vh|2vw|3vh|2vw|true|true” border_radii=”on|2vh|2vh|2vh|2vh” global_colors_info=”{}”]<\/p>\n

From early profiling to more relevant biological systems<\/strong><\/h3>\n

Once established, the probe can support target engagement studies across increasingly relevant experimental systems, helping you build a clearer picture of compound behaviour as your programme progresses.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.27.6″ _module_preset=”default” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.6″ _module_preset=”default” text_font=”|300|||||||” text_font_size=”28px” text_line_height=”1.4em” background_layout=”dark” global_colors_info=”{}”]Our work in tailored chemical proteomics for covalent inhibitors is supported by published work using the lysate-based approach.[\/et_pb_text][et_pb_button button_text=”Read more” _builder_version=”4.27.6″ _module_preset=”default” custom_button=”on” button_text_size=”18px” button_text_color=”#FFFFFF” button_bg_color=”#228CDB” button_border_width=”0px” button_border_radius=”5px” button_font=”Hanken||||||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/omivera.jppwebdesign.nl\/wp-content\/uploads\/2026\/09\/omi_Website-Omivera-60.png” title_text=”omi_Website Omivera (60)” _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Tailored chemical proteomics for covalent inhibitors Turn your covalent inhibitor into a chemical probe for target engagement and selectivity studies.Starting from your existing compound, Omivera can help develop a tailored chemical probe that enables you determine where in a biological system and which proteins your inhibitor engages. For a covalent inhibitor, a probe can be […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-427","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=\/wp\/v2\/pages\/427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=427"}],"version-history":[{"count":9,"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=\/wp\/v2\/pages\/427\/revisions"}],"predecessor-version":[{"id":697,"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=\/wp\/v2\/pages\/427\/revisions\/697"}],"wp:attachment":[{"href":"https:\/\/omivera.jppwebdesign.nl\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}